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A Report of the Witherspoon Council on Ethics and the Integrity of Science

The Stem Cell Debates: Lessons for Science and Politics

Appendices: A. The Science of Embryonic Stem Cell Research • B. The Promise of Stem Cell Therapies
C. Ethical Considerations Regarding Stem Cell Research • D. Stem Cell Research Funding: Policy and Law
E. Overview of International Human Embryonic Stem Cell Laws

The Promise of Stem Cell Therapies 

Appendix B

The Witherspoon Council

Table of Contents

There are two general categories of medical applications for stem cells: first, as an actual therapy, and second, as a way to model diseases to help researchers develop treatments. In this appendix, we discuss the therapeutic value of stem cells. A comprehensive analysis of every clinical treatment, study, and potential therapy is beyond the scope of this report. Instead, this sketch is intended to offer a realistic and up-to-date appraisal of this rapidly evolving field.

Many stem cell therapies are based on the regenerative capacities of stem cells to produce a variety of tissues, either in the patient’s body or in vitro.[1] Other therapies rely on transplanted stem cells, particularly adult mesenchymal stem cells (a type of multipotent stem cell), to provide signals that modify or regulate the activities of nearby cells without actually integrating into the patient’s tissues.[2] At present, treatments in regular clinical use are limited to adult stem cells, although clinical trials have begun for deriving induced pluripotent stem (iPS) cells from patients to help researchers study diseases,[3] and the U.S. Food and Drug Administration in 2010 approved the first clinical trials for therapies using human embryonic stem cells.[4] As of this writing, there are two FDA-approved clinical trials using human ES cells underway in the United States, one of which is also being performed in Europe; one additional FDA-approved trial was recently canceled (detailed below).[5]

There are several types of conditions that either are currently being treated with stem cell-based therapies or that hold out the prospect for such therapies in the future. These include autoimmune diseases, neurological disorders, cancers, and infertility. Furthermore, stem cells may be used in regenerative medicine to replace or repair tissues and organs damaged by disease or injury. Below, we discuss each of these categories in turn.

Autoimmune Diseases. Autoimmune diseases occur when the immune system mistakenly attacks tissues that are normally present in the body.[6] Some of the autoimmune diseases that stem cell therapies have been proposed for include multiple sclerosis, rheumatoid arthritis, juvenile idiopathic arthritis, Crohn’s disease, type 1 diabetes mellitus, autoimmune cytopenias, systemic lupus erythematosus, and systemic sclerosis.[7]

Clinical trials are now underway involving at least two distinct types of adult stem cell therapies directed at autoimmune diseases. The first is hematopoietic stem cell transplantation (HSCT). It is one of the best understood and most widely practiced forms of stem cell transplantation for restoring tissue function, and has been called the “gold standard” for the field by stem cell scientists.[8] In HSCT, hematopoietic stem cells — multipotent stem cells that produce many types of blood cell — are transplanted into the patient. Over the past decade, this technique has been used to treat a number of autoimmune diseases by first suppressing the patient’s immune system with high doses of chemotherapy or radiation and then transplanting the stem cells into the patient in an attempt to restore the immune system to normal function.[9] A subcategory of HSCT involves hematopoietic stem cells that are “autologous” — that is, they come not from donors but are harvested from the patient’s own body before the chemotherapy or radiation. Using the patient’s own stem cells avoids complications arising from immunological rejection of foreign tissues, as well as the inverse danger of “graft-versus-host disease,” wherein transplanted immune system cells attack tissues in the patient’s body.[10]

The ability of HSCT to restore the body’s blood-forming functions after high-dose chemotherapy has proven useful not only for the treatment of autoimmune diseases, but for many other blood-related disorders,[11] including leukemia (discussed further below). In these cases, the stem cell transplantation does not treat the disease itself, but rather alleviates the potentially severe side effects of high-intensity chemotherapy, allowing for higher doses of chemotherapy than would otherwise be possible.[12] (Advocates of human embryonic stem cell research, arguing against the claim that adult stem cells are a viable alternative to embryonic stem cells, have noted that chemotherapy, not adult stem cells per se, is the “primary treatment” in many of the therapies that involve HSCT.[13])

A second category of adult stem cell therapy that holds promise for treating autoimmune diseases is mesenchymal stem cell transplantation (MSCT). This involves transplanting into patients mesenchymal stem cells derived from bone marrow. In contrast to HSCT, which relies on chemotherapy or radiotherapy to suppress the immune system, in MSCT the mesenchymal stem cells themselves seem to suppress autoimmune responses.[14] Several clinical trials are underway to study the prospect of using MSCT therapy in the treatment of multiple sclerosis, Crohn’s disease, type 1 diabetes, systemic lupus erythematosus, systemic sclerosis, and Sjögren syndrome.[15] The ability of MSCT to modulate immune responses has also led researchers to believe it may be useful for treating graft-versus-host disease in transplant patients, and clinical trials are underway to test this ability.[16]

Autologous HSCT therapy has been attempted in patients with multiple sclerosis who have not responded to conventional therapies. The results so far have been mixed: the condition of some younger patients has stabilized, but the condition of other patients has deteriorated after transplantation.[17] MSCT therapy also holds out the prospect of treating multiple sclerosis, both by alleviating the condition through suppressing the autoimmune response and also by helping to heal damaged nerve cells by releasing signals that promote repair and regeneration.[18] It had been hoped that stem cells would allow for the growth of replacements for the oligodendrocytes and myelin sheaths damaged in patients with multiple sclerosis, but the prospects of such direct replacements using cells derived from bone marrow have since dimmed, and concerns about the tendency of embryonic stem cells to produce tumors has precluded clinical trials.[19] There is, however, some evidence that MSCT can promote repair in these tissues by indirect means, such as providing signals to the neural stem cells that are already present in the brain.[20]

Autologous HSCT has also shown promise in clinical trials in treating rheumatoid arthritis[21] and juvenile idiopathic arthritis.[22] And it has brought about remission of Crohn’s disease in patients whose condition had not responded to more conventional treatments.[23] Diabetes is another disease that in some forms is caused by autoimmune responses, and several studies have shown these forms responding positively to autologous HSCT. In these cases, the stem cell treatment has been shown to reduce the need for insulin — even, in combination with other therapies, allowing patients to forgo insulin injections.[24]

In a study that began in 2007 but has recently received increased media attention, scientists from Germany used HSCT to treat an HIV patient who was also suffering from acute myeloid leukemia. The treatment relied on finding a donor who possessed a mutation that conferred resistance to the virus. The patient first underwent chemotherapy to treat his leukemia; his immune system was suppressed as a side effect of the chemotherapy. Hematopoietic stem cells from the donor were transplanted to restore the patient’s immune system. After the transplant, the patient’s immune system cells were replaced by the HIV-immune cells produced by the transplanted stem cells, with complete replacement obtained 61 days after the transplantation. As a result of the treatment, the HIV virus is no longer detectable in the patient, indicating that the patient’s HIV infection may have been cured.[25]

Neurological Disorders. One common cause of loss of neural function is stroke. At least one study has shown that autologous MSCT can decrease neurological deficits in stroke patients.[26]

Still, while stem cells seem to offer a promising therapeutic option for stroke patients, several important problems remain unresolved, including choosing the right type of cells to derive from stem cells to use in transplantation, the number of cells to be transplanted, and the challenge of actually delivering stem cells to the damaged areas of the brain.[27] While the optimal method of delivering stem cells to the brain has presented some difficulty, researchers have found that transplanted neural stem cells seem able to migrate towards damaged tissues.[28]

Parkinson’s disease has been widely heralded as an area in which embryonic stem cells in particular may hold prospects for treatment. It has been suggested, for example, that neurons derived from ES cells might treat the disease when transplanted into a patient’s brain.[29] And one research team has recently shown that transplanted iPS cells can improve the condition of rats with Parkinson’s disease.[30]

Alzheimer’s disease was long thought not to be a promising candidate for stem cell therapy. However, in a study conducted with rats and mice, transplants of neural stem cells have migrated to damaged regions of the brain and improved synaptic connectivity among neurons by producing brain-derived neurotrophic factor, a compound that stimulates the development of synaptic connections.[31] In a study on rats that had been injured to simulate Alzheimer’s disease, neural precursor cells derived in vitro from ES cells were shown to improve cognitive functioning in the rats. While the researchers observed that the neural precursor cells differentiated into neuron-like cells in vivo, they noted that the therapeutic results of the treatment were likely due not to the replacement of neurons but rather to the release of neurotrophic factors.[32]

Researchers have also used embryonic stem cells to generate what appear to be one of the important cell types that is lost during Alzheimer’s disease: basal forebrain cholinergic neurons. This could be useful as a way to study the disease, as well as offering a potential source of neurons for cell-transplantation therapy.[33]

Cancer. A number of applications for stem cell therapies in treating cancer have been found. Hematopoietic stem cells employed through bone marrow transplantations have been used since the 1950s to treat leukemia, a form of cancer that affects the blood and bone marrow.[34] There is growing evidence that HSCT can be an effective treatment for other forms of cancer as well. Originally, doctors used HSCT in conjunction with high doses of chemotherapy. The aggressive chemotherapy regimen would destroy resistant tumors in the bone marrow, but it could also cause lethal damage to the patient’s blood-forming and immune systems, requiring HSCT to restore these systems to their normal functions.[35] Some researchers have raised questions about whether employing autologous HSCT under this strategy achieves better results when treating metastatic breast cancer than conventional chemotherapy without autologous HSCT.[36]

Also, some researchers believe that one of the troublesome effects of HSCT using donor stem cells, the graft-versus-host problem, can actually be harnessed to fight cancer. There is evidence that HSCT using donor stem cells can stimulate an immune response against the cancer cells in the patient’s body — a “graft-versus-leukemia” or “graft-versus-tumor” effect.[37] To take advantage of that effect, some researchers have suggested that regimens of highly toxic chemotherapy might be replaced by less aggressive chemotherapy accompanied by HSCT.[38] While this technique was pioneered for the treatment of leukemia, doctors have begun to explore the possibility that there is a more general “graft-versus-cancer” effect, using HSCT to treat “metastatic colon carcinoma, ovarian carcinoma, advanced pancreatic carcinoma, prostate cancer and neuroblastoma.”[39]

Reproductive Applications. There is evidence that both ES[40] and iPS[41] cells have the ability to form primordial germ cells — the cells from which gametes (eggs and sperm) are generated. The results of recent experiments in animals have led some researchers in reproductive medicine to suggest that generating sperm from ES cells may be a promising treatment for severe male infertility.[42] But other research has shown the challenges facing such treatments: mice born from stem cell-derived gametes died shortly after birth due to congenital defects.[43] It is worth noting that if gametes created from ES cells are used to create a new organism, that organism will have at least one genetic parent that is a destroyed embryo.

Additionally, the reprogramming techniques that already allow researchers to induce somatic cells into a pluripotent state might someday, at least theoretically, be adapted to reprogram somatic cells to a totipotent stage. This would possibly allow for human cloning without the use of eggs, since totipotent stem cells would be quite similar to embryos that could be implanted in the womb.[44] (Creating embryos in this manner would face considerable technical hurdles, however. The human egg cell is considerably larger than ordinary human cells, and is specially adapted for becoming an embryo upon fertilization, containing a large volume of cellular and genetic factors that are essential for development. Even if reprogramming were to deliver the right types of genetic factors to an adult cell, it is not clear that the reprogrammed cell would be capable of the kind of embryonic development that is characteristic of fertilized egg cells.)

Furthermore, iPS and ES cells could both also be used for creating offspring through a stem cell-based cloning technique called tetraploid complementation. In this procedure, pluripotent stem cells are injected into a modified embryo that provides the placental layer but does not contribute to the development of the embryo itself, which develops directly from the pluripotent stem cells.[45] This allows researchers to create an organism that is genetically identical to a pluripotent stem cell.[46] (The genetic “parent” of an organism created through this procedure will be the single individual from whom the pluripotent stem cells were derived — either a destroyed embryo or an adult organism, depending on whether an ES cell or an iPS cell is used. And the modified “tetraploid” embryo that provides the placental layer will have a novel relationship with the child: it would not contribute to the child genetically but would play an indispensable role in the child’s development that is not comparable to any natural biological relationship.)

As mentioned in Appendix A of this report, tetraploid complementation is already regularly used to analyze animal stem cells.[47] It has also been used for nearly twenty years to clone mice for research purposes, often from stem cells that have been genetically modified in vitro to produce genetically engineered organisms. While tetraploid complementation has never been performed on primates for practical and ethical reasons, some experts argue that, in principle, it could eventually be performed on humans.[48] The technique was performed on mice years before the controversy surrounding Dolly the cloned sheep, though it has received very little public attention — perhaps because, until recently, the only source of pluripotent stem cells were early-stage embryos, so the procedure could not have been used to clone an adult organism. However, with the advent of iPS cell technology, it is now possible to derive pluripotent stem cells from adult organisms, meaning that this procedure could possibly be used to clone mature organisms.[49] Indeed, in 2009, researchers performed a successful tetraploid complementation experiment using iPS cells from mice.[50] However, because it has not been attempted in primates with either embryonic or induced pluripotent stem cells, it is an open question as to whether this technique will ever be able to clone human beings.

Regenerative Medicine: Organ and Tissue Repair and Replacement. One of the most therapeutically promising prospects of stem cell research has been the possibility of repairing or replacing damaged organs and tissues — that is, of replicating the generative process that normally takes place only in utero. While ES cells have shown a great deal of potential in this area, owing to their pluripotency — their ability to develop into a wide variety of tissue types — there have been some successes toward using adult stem cells as well. For example, in 2008, adult stem cells were used to create a new trachea for a woman in her early thirties.[51] All the tissue was removed from a trachea procured from a deceased donor, leaving behind a bare scaffolding of cartilage. Scientists then seeded that scaffolding with the woman’s own mesenchymal stem cells; the resulting trachea was successfully transplanted into the patient, with no noted complications from tissue rejection. Even more recently, scientists have used stem cells to create an artificial trachea — one with stem cells seeded upon a scaffolding of plastic rather than cartilage.[52] There have also been at least three trials using autologous mesenchymal stem cells to repair damaged cartilage, all of which showed promise.[53]

Additionally, doctors have recently used hematopoietic stem cells to successfully culture human red blood cells in vitro, which they used in blood transfusions. The cultured red blood cells were able to survive and mature into fully functioning cells in the patients’ bloodstreams, demonstrating the potential of these cells to serve as an alternative to conventional blood donation. In order to cultivate these cells in vitro, researchers needed to find the right mix of growth factors that would coax the hematopoietic stem cells to successfully differentiate. While this represents a major breakthrough in stem cell therapy that will surely be beneficial to many patients in need of blood transfusions, the fact that we have only recently been able to use stem cells to regenerate red blood cells is indicative of the challenges facing the development of in vitro tissue and organ regeneration.

One of the most common causes of heart failure is ischemic heart disease, which results from damage to heart tissue. Treating ischemic heart disease by repairing this damage represents one of the major goals of regenerative medicine. One trial, involving several research teams, performed transplantations of autologous myoblasts, or muscle stem cells, on patients recovering from heart attacks, but showed results no better than with transplanted placebos.[54] However, another recent study showed a promising treatment of damaged heart muscle with iPS cell transplants.[55] Heart muscle cells derived from human ES cells have also been shown to have some promise as treatments in animal models.[56] Perhaps the most promising results in this field have come from a recent study of sixteen patients treated with autologous cardiac stem cells. The therapy appeared to regenerate the damaged heart tissue and significantly improve heart function in patients.[57]

Macular degeneration, a condition that can lead to loss of vision, is another disease for which stem cell therapy is thought to hold promise. In November 2010, the FDA granted approval to Advanced Cell Technologies (ACT) to perform a clinical trial assessing the safety of ES cell-derived treatments for Stargardt disease, a form of macular degeneration that affects children,[58] and in January 2011, the FDA approved another ACT clinical trial testing the same treatment for dry age-related macular degeneration, a condition that can lead to blindness in people over the age of 55.[59] On July 14, 2011, the company announced that it had, as part of those clinical trials, transplanted retinal pigment epithelial cells derived from human ES cells into two patients without any safety complications.[60] On September 22, 2011, the U.K. Medicines and Healthcare Products Regulatory Agency approved an ACT clinical trial for treatment of Stargardt disease, marking the first European embryonic stem cell trial.[61]

The holy grail of regenerative medicine is the treatment of spinal cord injuries. No other treatment for these devastating injuries has appeared in recent decades, and the plight of Christopher Reeve attracted a great deal of attention to the possibility that stem cell therapy might someday reverse such injuries. However, treatments by embryonic, adult, and induced pluripotent stem cells have all faced significant technical challenges.[62] The first clinical trial in human beings involving human embryonic stem cells was a study conducted by the Geron Corporation, a biotechnology company; the study was approved by the FDA in January 2009 and it commenced in October 2010.[63] The subject of the study had suffered a spinal cord injury and was injected with oligodendrocyte progenitor cells derived from human embryonic stem cells.[64] However, in November 2011, Geron announced that it was canceling its stem cell program.[65] The company said that, while it will not be enrolling any new patients in the therapy, it will continue to follow currently enrolled patients and to update the FDA and the medical community on their progress.[66] The company reported that the therapy had been “well tolerated with no serious adverse events.”[67]

Unapproved Therapies and Stem Cell Tourism. Both adult and embryonic stem cells hold great therapeutic promise, but it is important to remember that stem cell science and medicine are still new fields, and that much more work will be needed before safe, effective therapies can become widely available. While stem cells offer a powerful therapeutic tool in some circumstances, they also can come with considerable risks. The FDA has been reluctant to license stem cell therapies in the United States because of the insufficient evidence of their safety and efficacy.[68]

Some proponents of adult stem cell therapy have argued that because autologous stem cell transplantations employ the patient’s own tissues, they should not be considered “drugs,” and should therefore be subject only to the self-regulation of medical practitioners.[69] Many countries, particularly China and various European states, have more permissive policies on adult stem cell treatments than the United States, leading many Americans to go abroad for stem cell therapies not approved by the FDA — a practice known as “stem cell tourism.” Experimental stem cell treatments offer hope to patients with serious medical conditions, including patients who have exhausted all conventional therapies, and stem cell clinics often employ direct-to-consumer advertisements that take advantage of the vulnerability and desperation of the ailing.[70] Several high-profile American celebrities have undergone unapproved experimental treatments, sometimes at clinics overseas, which may lend further seeming credibility to these unproven treatments.[71]

But many stem cell scientists and agencies have warned patients of the dangers posed by clinics claiming to offer unproven stem cell therapies for a variety of conditions.[72] One prominent critic, Douglas Sipp of the RIKEN Center for Developmental Biology, has counted more than two hundred clinics around the world “offering some version of stem cells for some type of medical condition for which there’s no really good evidence that stem cells would be either safe or effective.”[73] “When someone advertises stem cell products for the treatment of conditions like ALS, spinal-cord injury, Down syndrome, autism, or any of the scores of other unfounded claims that have flooded the Internet,” Sipp has written, it is “unscientific, deceitful, and predatory” and deserves “serious monitoring and regulatory involvement.”[74]

Conclusion

Stem cells play a fundamental role in our biological development and they promise to provide medical science with a powerful tool. Scientists can use pluripotent stem cells to create specific human tissues in the laboratory to serve as models for studying how diseases develop or as platforms for testing new drugs and treatments. And, as the brief survey above has shown, both multipotent and pluripotent stem cells hold out hope for treatments for many of the conditions that afflict the body.

However, it is important not to overlook the fact that several of the therapies described above are still experimental and may ultimately prove to be ineffective or impractical. It is also important to remember that powerful therapeutic tools have significant costs and difficulties associated with them — not only the financial costs of their development and application, but also the risks associated with their use. The conditioning regimens for HSCT, for instance, can be very painful and dangerous, involving high doses of chemotherapy and total-body irradiation to destroy tumors or other malignant blood conditions. As with other forms of transplantation, stem cell transplants can result in dangerous immune reactions, and avoiding those immune reactions by creating personalized stem cell lines poses numerous practical and technical challenges. Significant hurdles would also have to be surmounted before nuclear transfer (either SCNT or ANT) could ever be a practical medical tool, given the complexities of the procedure and the difficulty of procuring the large number of human egg cells that it requires. Likewise, iPS cell techniques have not yet reached the point where they can reliably produce safe, effective pluripotent stem cells. And transplanted pluripotent cells continue to pose a cancer risk that researchers have barely begun to explore. While the power of stem cells to treat diseases holds great promise, we must remember that many of these therapies remain unproven and may come at a considerable cost.

In the next appendix, we turn to the ethical implications of research and treatment involving stem cells.



Notes
[1] Daley George and David T. Scadden, “Prospects for Stem Cell Therapy,” Cell 132, no. 4 (2008): 544-548.
[2] Ibid.
[3] NIH Clinical Trials website, http://clinicaltrials.gov/ct2/results?term=ips+cell (accessed July 30, 2011).
[4] Geron, “Geron Receives FDA Clearance to Begin World’s First Human Clinical Trial of Embryonic Stem Cell-Based Therapy,” press release, January 23, 2009, http://geron.com/media/pressview.aspx?id=1148.
[5] Jocelyn Kaiser, “FDA Lifts Hold on First-Ever Embryonic Stem Cell Trial,” Science Insider, July 30, 2010, http://news.sciencemag.org/scienceinsider/2010/07/fda-lifts-hold-on-first-ever-embryonic.html; Geron, “Geron to Proceed with First Human Clinical Trial of Embryonic Stem Cell-Based Therapy,” press release, July 30, 2010, http://www.geron.com/media/pressview.aspx?id=1229; Advanced Cell Technology, Inc. (ACT), “Advanced Cell Technology Receives FDA Clearance For the First Clinical Trial Using Embryonic Stem Cells to Treat Macular Degeneration,” press release, November 22, 2010, http://www.advancedcell.com/news-and-media/press-releases/advanced-cell-technology-receives-fda-clearance-for-the-first-clinical-trial-using-embryonic-stem-cel/index.asp; ACT, “Advanced Cell Technology Receives FDA Clearance For Clinical Trials Using Embryonic Stem Cells to Treat Age-Related Macular Degeneration,” press release, January 3, 2011, http://advancedcell.com/news-and-media/press-releases/advanced-cell-technology-receives-fda-clearance-for-clinical-trials-using-embryonic-stem-cells-to-tre/; ACT, “ACT Receives Approval for First Human Embryonic Stem Cell Trial in Europe,” press release, September 22, 2011, http://www.advancedcell.com/news-and-media/press-releases/act-receives-approval-for-first-human-embryonic-stem-cell-trial-in-europe/index.asp; Gretchen Vogel, “U.K. Approves Europe’s First Embryonic Stem Cell Clinical Trial,” Science Insider, September 22, 2011, http://news.sciencemag.org/scienceinsider/2011/09/uk-approves-europes-first-embryonic.html.
[6] Neil A. Campbell and Jane Reece, Biology, 7th ed. (San Francisco: Pearson Education, 2005), 917.
[7] Daniele Lodi, Tommaso Iannitti, and Beniamino Palmieri, “2011 Stem Cells in Clinical Practice: Applications and Warnings,” Journal of Experimental & Clinical Cancer Research 30, no. 9 (2011): 1062-1070.
[8] Daley and Scadden, “Prospects for Stem Cell-Based Therapy,” 544-548.
[9] Alan Tyndall and Alois Gratwohl, “Adult Stem Cell Transplantation and Autoimmune Disease,” Current Opinion in Hematology 16, no. 4 (2009): 285-291.
[10] Douglas A. Hale, “Basic Transplantation Immunology,” Surgical Clinics of North America 86, no. 5 (2006): 1103-1125.
[11] Peter A. McSweeney and Rainer Storb, “1998 Hematopoietic Stem Cell Transplantation,” in Encyclopaedia of Immunology, ed. Peter J. Delves and Ivan M. Roitt (San Diego: Academic Press, 1998), 1062-1070.
[12] R. K. Burt et al., “Collection of Hematopoietc Stem Cells from Patients with Autoimmune Diseases,” Bone Marrow Transplantation 28, no. 1 (2001): 1-12.
[13] Shane Smith, William Neaves, and Steven Teitelbaum, “Adult Versus Embryonic Stem Cells: TreatmentsScience 316, no. 5830 (2007): 1422-1423.
[14] Antonio Uccelli, Lorenzo Moretta, and Vito Pistoia, “Immunoregulatory Function of Mesenchymal Stem Cells,” European Journal of Immunology 36, no. 10 (2006): 2566-2573.
[15] Antonio Uccelli and Darwin J. Prockop, “Why Should Mesenchymal Stem Cells (MSCs) Cure Autoimmune Diseases?,” Current Opinion in Immunology 22, no. 6 (2010): 768-774.
[17] A. S. Fassas et al., “Hematopoietic Stem Cell Transplantation for Multiple Sclerosis: A Retrospective Multicenter Study,” Journal of Neurology 249, no. 8 (2002): 1088-97.
[19] N. Scolding, “Adult Stem Cells and Multiple Sclerosis,” Cell Proliferation 44 (Supplement s1 to April 2011): 35-38.
[20] Ibid.
[21] John A. Snowden et al., “Autologous Hemopoietic Stem Cell Transplantation in Severe Rheumatoid Arthritis: A Report from the EBMT and ABMTR,” Journal of Rheumatology 31, no. 3 (2004): 482-488.
[23] Yu Oyama et al., “Autologous Hematopoietic Stem Cell Transplantation in Patients with Refractory Crohn’s Disease,” Gasteroenterology 128, no. 3 (2005): 552-563.
[24] Julia C. Voltarelli et al., “Autologous Nonmyeloablative Hematopoietic Stem Cell Transplantation in Newly Diagnosed Type 1 Diabetes Mellitus,” Journal of the American Medical Association 297, no. 14 (2005): 1568-1576.
[25] Gero Hutter et al., “Long-Term Control of HIV by CCR5 Δ32/Δ32 Stem-Cell Transplantation,” The New England Journal of Medicine 360, no. 7 (2009): 692-698; Kristina Allers et al., “Evidence for the Cure of HIV Infection by CCR5Δ32/Δ32 Stem Cell Transplantation,” Blood 117, no. 10 (2011): 2791-2799.
[26] Oh Young Bang et al., “Autologous Mesenchymal Stem Cell Transplantation in Stroke Patients,” Annals of Neurology 57, no. 6 (2005): 874-882.
[27] Soma Banerjee et al., “Human Stem Cell Therapy in Ischaemic Stroke: A Review,” Age and Aging 40, no. 1 (2011): 7-13.
[29] Myung Soo Cho, Dong Youn Hwang, and Dong Wook Kim, “Efficient Derivation of Functional Dopaminergic Neurons from Human Embryonic Stem Cells on a Large Scale,” Nature Protocols 3, no. 12 (2008): 1888-1894.
[31] Mathew Blurton-Jones et al., “Neural Stem Cells Improve Cognition via BDNF in a Transgenic Model of Alzheimer Disease,” PNAS 106, no. 32 (2009): 13594-13599.
[32] Jun Tang et al., “Embryonic Stem Cell-Derived Neural Precursor Cells Improve Memory Dysfunction in Aβ(1-40) Injured Rats,” Neuroscience Research 62, no. 2 (2008): 86-96.
[33] Christopher J. Bissonnette et al., “The Controlled Generation of Functional Basal Forebrain Cholinergic Neurons from Human Embryonic Stem Cells,” Stem Cells 29, no. 5 (2011): 802-811.
[34] E. Donnall Thomas et al., “Intravenous Infusion of Bone Marrow in Patients Receiving Radiation and Chemotherapy,” The New England Journal of Medicine 257, no. 11 (1957): 491-496.
[35] Keren Osman and Raymond L. Comenzo, “Stem Cell Transplantation,” in Encyclopedia of Cancer, 2nd ed., ed. Joseph R. Bertino (San Diego: Academic Press, 2002), 1:253-263.
[37] Mary M. Horowitz et al., “Graft-Versus-Leukemia Reactions after Bone Marrow Transplantation,” Blood 75, no. 3 (1990): 555-562; Cornelius Schmaltz and Marcel R. M. van den Brink, “Graft versus Leukemia and Graft versus Tumor Activity,” in Encyclopedia of Cancer, 2nd ed., ed. Joseph R. Bertino (San Diego: Academic Press, 2002), 1:325-338.
[38] Shimon Slavin et al., “Treatment of Leukemia by Alloreactive Lymphocytes and Nonmyeloablative Stem Cell Transplantation,” Journal of Clinical Immunology 22, no. 2 (2002): 64-69.
[39] Olle Ringden et al., “The Allogeneic Graft-Versus-Cancer Effect,” British Journal of Haematology 147, no. 5 (2009): 614-633.
[40] Amander T. Clark et al., “Spontaneous Differentiation of Germ Cells from Human Embryonic Stem Cells In Vitro,” Human Molecular Genetics 13, no. 7 (2004): 727-739.
[41] Sarita Panula et al., “Human Germ Cell Differentiation from Fetal- and Adult-derived Induced Pluripotent Stem Cells,” Human Molecular Genetics 20, no. 4 (2010): 752-762.
[42] Laura Rienzi et al., “What to Do When ICSI Fails,” Systems Biology in Reproductive Medicine 56, no. 5 (2010): 376-387.
[43] Andrew J. Childs, Phillipa T. K. Saunders, and Richard A. Anderson, “Modelling Germ Cell Development In Vitro,” Molecular Human Reproduction 14, no. 9 (2008): 501-511; Bernard A. J. Roelen and Susana M. Chuva de Sousa Lopes, “Of Stem Cells and Gametes: Similarities and Differences,” Current Medicinal Chemistry 15, no. 13 (2008): 1249-1256.
[44] President’s Council on Bioethics, Alternative Sources of Pluripotent Stem Cells, Washington, D.C., 2005. See, for example, pp. 5 and 50.
[45] András Nagy et al., “Derivation of Completely Cell Culture-Derived Mice from Early-Passage Embryonic Stem Cells,” PNAS 90, no. 18 (1993): 8424-8428.
[46] Ibid.
[47] Lan Kang et al., “iPS Cells Can Support Full-term Development of Tetraploid Blastocyst-Complemented Embryos,” Cell Stem Cell 5, no. 2 (2009): 135-138.
[48] Hans-Werner Denker, “Human Embryonic Stem Cells: The Real Challenge for Research as Well as for Bioethics Is Still Ahead of Us,” Cells Tissues Organs 187, no. 4 (2008): 250-256.
[49] Lan Kang et al., “iPS Cells Can Support Full-term Development of Tetraploid Blastocyst-Complemented Embryos,” 135-138.
[50] Ibid.
[51] Augustinus Bader and Paolo Macchiarini, “Moving Towards In Situ Tracheal Regeneration: The Bionic Tissue Engineered Transplantation Approach,” Journal of Cellular and Molecular Medicine 14, no. 7 (2010): 1877-1889; Paolo Macchiarini et al., “Clinical Transplantation of a Tissue-Engineered Airway,” The Lancet 372, no. 9655 (2008): 2023-2030.
[52] Gautam Naik, “Lab-Made Trachea Saves Man,” Wall Street Journal, July 8, 2011, http://on.wsj.com/pFq3yJ.
[55] Timothy J. Nelson et al., “Repair of Acute Myocardial Infarction by Human Stemness Factors Induced Pluripotent Stem Cells,” Circulation 120, no. 5 (2009): 408-416.
[57] Roberto Bolli et al., “Cardiac Stem Cells in Patients with Ischaemic Cardiomyopathy (SCIPIO): Initial Results of a Randomised Phase 1 Trial,The Lancet, early online publication, November 14, 2011.
[58] Advanced Cell Technology, “Advanced Cell Technology Receives FDA Clearance For the First Clinical Trial Using Embryonic Stem Cells to Treat Macular Degeneration,” press release, November 22, 2010, http://www.advancedcell.com/documents/0000/0272/FDA_Approval_Press_Release_Monday.pdf.
[59] Advanced Cell Technology, “Advanced Cell Technology Receives FDA Clearance For Clinical Trials Using Embryonic Stem Cells to Treat Age-Related Macular Degeneration,” press release, January 3, 2011, http://www.advancedcell.com/news-and-media/press-releases/advanced-cell-technology-receives-fda-clearance-for-clinical-trials-using-embryonic-stem-cells-to-tre/.
[60] Advanced Cell Technology, “ACT Announces First Patients Undergo Embryonic Stem Cell Transplantation Treatment for Stargardt’s Disease and Macular Degeneration at UCLA’s Jules Stein Eye Institute,” press release, July 14, 2011, http://www.advancedcell.com/news-and-media/press-releases/act-announces-first-patients-undergo-embryonic-stem-cell-transplantation-treatment-for-stargardts-disease-and-macular-degeneration-at-uclas-jules-stein-eye-institute/index.asp.
[61] Rachael Panizzo, “Britain Trials Embryonic Stem Cells as Treatment for Blindness,” BioNews, September 26, 2011, http://www.bionews.org.uk/page_107183.asp.
[62] Enci Mary Kan, Eng-Ang Ling, and J. Lu, “Stem Cell Therapy for Spinal Cord Injury,” Current Medicinal Chemistry 17, no. 36 (2010): 4492-4510.
[63] Geron, “Geron Receives FDA Clearance to Begin World’s First Human Clinical Trial of Embryonic Stem Cell-Based Therapy,” press release, January 23, 2009, http://geron.com/media/pressview.aspx?id=1148.
[64] Geron, “Geron Initiates Clinical Trial of Human Embryonic Stem Cell-Based Therapy,” press release, January 23, 2009, http://www.geron.com/media/pressview.aspx?id=1235.
[65] Geron, “Geron to Focus on its Novel Cancer Programs,” press release, November 14, 2011, http://www.geron.com/media/pressview.aspx?id=1284.
[66] Ibid.
[67] Ibid.
[68] Eliza Barclay, “Stem-cell Experts Raise Concerns About Medical Tourism,” The Lancet 373, no. 9667 (2009): 883-884.
[69] American Stem Cell Therapy Association, “Physician Group Opposes FDA’s Position on Adult Stem Cells,” press release, March 26, 2009, PRWeb, http://www.prweb.com/releases/ASCTA/stemcelltherapy/prweb2264534.htm; Zubin Master and David B. Resnik. “Stem-cell Tourism and Scientific Responsibility,” EMBO Reports 12 (2011): 1-4.
[70] Barclay, “Stem-cell Experts Raise Concerns About Medical Tourism,” 883-884.
[71] Governor Rick Perry of Texas; NFL players Peyton Manning, Terrell Owens, and Jarvis Green; Yankees pitcher Bartolo Colón; golfer John Daly; and musicians Don Ho and Tony Iommi are among the celebrities who have reportedly undergone stem cell treatments unapproved by the FDA. “Doctors Wary of Perry’s Stem Cell Treatment,” Boston.com, August 20, 2011, http://articles.boston.com/2011-08-20/news/29909865_1_cell-treatment-unproven-treatments-governor-rick-perry; Rachael Rettner, “Peyton Manning’s Treatment: How Safe Is It?” MSNBC.com, September 21, 2011, http://www.msnbc.msn.com/id/44610314/; Robert Klemko, “Report: Terrell Owens in South Korea for Stem-cell Treatment,” USA Today, September 20, 2011, http://content.usatoday.com/communities/thehuddle/post/2011/09/report-terrell-owens-in-south-korea-for-stem-cell-treatment/1; Albert Breer, “Green’s Stem-cell Success Story Bodes Well for Manning,” NFL Network, September 23, 2011, http://www.nfl.com/news/story/09000d5d8227e0ce/article/greens-stemcell-success-story-bodes-well-for-manning; Serge F. Kovaleski, “Pitcher’s Treatment Draws Scrutiny,” New York Times, May 11, 2011, http://www.nytimes.com/2011/05/12/sports/baseball/disputed-treatment-was-used-in-bartolo-colons-comeback.html?pagewanted=all; Dan Baynes, “Golfer Daly to Return Ahead of Schedule After Stem-Cell Surgery,” Bloomberg.com, October 13, 2009, http://www.bloomberg.com/apps/news?pid=newsarchive&sid=aaMDdL6Byy9s; “Don Ho Recovering from Stem Cell Procedure,” Associated Press, December 6, 2005, http://www.usatoday.com/life/people/2005-12-06-don-ho-stem-cell_x.htm; Tim Masters, “Ionni Says Injured Hand on Mend,” BBC News, November 3, 2009, http://news.bbc.co.uk/2/hi/entertainment/8340697.stm.
[72] Monya Baker, “Stem Cell Society Condemns Unproven Treatments,” Nature Reports Stem Cells, June 26, 2008, http://www.nature.com/stemcells/2008/0806/080626/full/stemcells.2008.98.html; International Society for Stem Cell Research, “A Closer Look at Stem Cell Treatments,” http://www.closerlookatstemcells.org/Stem_Cell_Treatment.htm; Danielle Nicholson, “Interview with Doug Sipp, Manager at RIKEN CDB, Kobe, Japan,” EuroStemCell, August 17, 2011, http://www.eurostemcell.org/commentanalysis/interview-doug-sipp-manager-riken-cdb-kobe-japan.
[73] “21st Century Snake Oil,” 60 Minutes, April 18, 2010, available at http://www.youtube.com/watch?v=Rb2NBlmL1es.
[74] Doug Sipp, “Stem Cell Pseudoscience: Calling a Spade a Spade,” Stem Cell Treatments Monitor (blog), September 5, 2011, http://sctmonitor.blogspot.com/2011/09/stem-cell-pseudomedicine-calling-spade.html.

The Witherspoon Council on Ethics and the Integrity of Science, "Appendix B: The Promise of Stem Cell Therapies," The New Atlantis, Number 34, Winter 2012, pp. 82-97.